T3253
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Useful Links & Tools
- MSDS
- Specification Sheet
- Certificate of Analysis
- Certificate of Origin
- TRIZMA 106B Bulletin
- T3253 - Product Information Sheet (52KB)
- FT-IR Condensed Phase
- FT-IR Raman
T3253
Sigma
Trizma® hydrochloride
reagent grade, ≥99.0% (redox titration), crystalline
| Synonym: | TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride |
| CAS Number: | 1185-53-1 |
| Linear Formula: | NH2C(CH2OH)3 · HCl |
| Molecular Weight: | 157.60 |
| Beilstein Registry Number: | 3675235 |
| EC Number: | 214-684-5 |
| MDL number: | MFCD00012590 |
| PubChem Substance ID: | 24900119 |
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Application | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Other Notes | Easily compare specifications for Trizma HCl products with the Trizma HCl specification table. |
| Legal Information | Trizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | reagent grade |
| assay | ≥99.0% (redox titration) |
| form | crystalline |
| total impurities | ≤0.5% water (Karl Fischer) |
| color | clear colorless (40 % (w/w) in H2O) solution |
| white powder | |
| useful pH range | 7.0 - 9.0 |
| pKa (25 °C) | 8.1 |
| EQP level | Elite |
| mp | 150-152 °C |
| cation traces | heavy metals (as Pb): ≤5 ppm |
| abs. | A |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Gloves |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 1 |
References
| Beilstein | Beil. 4,H,304 |
